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The introduced Nde I and Eco RI restriction sites were used to clone the gene into the pPT-plasmid resulting in pPT_ roGFP2 coding for a TorA_roGFP2 fusion protein

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This zonation is essential for the selective transport of molecules across the BBB, with arterial ECs enriched in transcription factors and capillary and venous ECs predominantly express transporter genes

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