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E0771 cells were cultured in RPMI-1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 U mL −1 penicillin, and 100 μg mL −1 streptomycin (SV30010

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30 µg of protein was loaded onto an SDS-polyacrylamide gel (4% stacking, 10% separation density)